RNA Extraction Free/ FastPlex SARS-CoV-2 & Influenza A/B Detection Kit (RT-PCR)-
A one-step reverse transcription-polymerase chain reaction (RT-PCR) combined with Taqman hydrolysis probe technology was used to detect the RNAs in the sample by a fluorescence quantitative PCR instrument with changes in fluorescence signal.The PCR detection kit includes endogenous internal standard primers and probes and monitors the sample collection and extraction process by detecting whether the internal standard is normal to avoid false-negative results.
- FastPlex SARS-CoV-2 and Flu A&B Multiplex Detection Kit is a fast, highly sensitive multiplex test solution.
- It consists of both the assays and controls, which are needed for the real-time PCR RNA detection from the SARS-CoV-2 and Flu virus.
- It is a multiplex RT-PCR test kit, enabling quick and precise identification of different virus infection.
- One tube detects Influenza A, influenza B and SARS-CoV-2 simultaneously.
- FAM channel detects SARS-CoV-2, HEX channel detects Influenza A, Cy5 channel detects Influenza B,ROX channel detects Human internal reference gene as quality control
- Compatible with most 96 well plate real time fluorescence PCR instruments < 250 copies/ml LoD for flu A, flu B and COVID-19 virus
- Less than 90 minutes sample to results
- Best screening tool during flu season and COVID-19 pandemic
|Target Virus:||Flu A, Flu B and SARS-CoV-2|
|Method of Detection:||RealTime RT-PCR|
|Fluorescent Channels||FAM, HEX, Cy5, ROX|
|Compatible Sample Types:||Broncheoalveolar lavage; Throat swab|
|Applicable Instrument:||ABI 7500™; Bio-Rad CFX96™PCR systems|
|PCR Detection Buffer||Primers, Probes, dNTPs, MgCl2, PCR buffer||672μl×1||1 tube|
|Enzyme Mix||RT Enzyme, RNasin, Taq Enzyme||96μl×1||1 tube|
|Positive Control||Gblocks for ORF1ab, N gene and Human RNA for internal control RNase P gene||500µl x1||1 tube|
|Negative Control||Nuclease-free water||500μl×1||1 tube|